Xyloglucan Endotransglycosylase Activity in Pea Internodes [Effects of Applied Gibberellic Acid]

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Xyloglucan endotransglycosylase activity in pea internodes. Effects of applied gibberellic acid.

Xyloglucan endotransglycosylase (XET) activity extractable from internodes of tall and dwarf varieties of pea (Pisum sativum L.) was assayed radiochemically using tamarind seed xyloglucan as donor substrate and an oligosaccharidyl-[3H]alditol as acceptor substrate. Internodes I and II showed little elongation during the period 15 to 21 d after sowing; XET activity remained relatively constant a...

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Increased ribonucleic Acid polymerase activity associated with chromatin from internodes of dwarf pea plants treated with gibberellic Acid.

Gibberellic acid increases the level of RNA polymerase associated with chromatin isolated from expanding internodes of light-grown, dwarf pea plants (Pisum sativum L.), without a detectable increase in the amount of DNA template available.

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Promotion of growth and invertase activity by gibberellic Acid in developing Avena internodes.

Gibberellic acid (GA(3)) induces invertase activity within 6 hours in Avena stem segments that are incubated in the dark at 23 degrees . The maximum amount of promotion is about 5 times that of invertase activity in untreated segments. GA(3) causes significant promotion of invertase activity at concentrations as low as 3 x 10(-5) mum GA(3). The increase in invertase activity elicited by GA(3) b...

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Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants.

1. Cell-free extracts of all plants tested contained a novel enzyme activity (xyloglucan endotransglycosylase, XET) able to transfer a high-Mr portion from a donor xyloglucan to a suitable acceptor such as a xyloglucan-derived nonasaccharide (Glc4Xyl3GalFuc; XG9). 2. A simple assay for the enzyme, using [3H]XG9 and based on the ability of the [3H]polysaccharide product to bind to filter paper, ...

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lncreased Activity of Xyloglucan Endotransglycosylase and Its Possible Regulation by Abscisic Acid

Previous work suggested that an increase in cell wall-loosening contributes to the maintenance of maize (Zea mays 1.) primary root elongation at low water potentials (h). It was also shown that root elongation at low +w requires increased levels of abscisic acid (ABA). In this study we investigated the effects of low +w and ABA status on xyloglucan endotransglycosylase (XET) adivity in the root...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1993

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.103.1.235